| Primer | Species | LP | RP | Tml | Tmr | Length | Score | Target | Senstive |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Thermotoga petrophila | TGGTGATGACCGCAAGTCC | AGGTTCTGTGCGATCGTCC | 60.00 | 59.78 | 199 | 149 |
100.00%
|
50.00%
|
| Primer 2 | Thermotoga petrophila | TCTCGAGCGAGAACCCGAT | AGGGGATTGGGGAGGAGAT | 60.45 | 58.95 | 261 | 141 |
100.00%
|
100.00%
|
| Primer 3 | Thermotoga petrophila | TGGTGATGACCGCAAGTCC | TTCTGTGCGATCGTCCCAC | 60.00 | 60.08 | 196 | 136 |
100.00%
|
50.00%
|
| Primer 4 | Thermotoga petrophila | AAGCTCGAGAAAGCCCTGG | AGTGCAAAGCGGTGAAGC | 59.70 | 58.97 | 251 | 132 |
100.00%
|
100.00%
|
| Primer 5 | Thermotoga petrophila | TGTGTGTTGCTCATCCCGAT | AGGGGATTGGGGAGGAGAT | 59.68 | 58.95 | 232 | 130 |
100.00%
|
100.00%
|
| Primer 6 | Thermotoga petrophila | AGACACGCACACGCATCT | ACTTCTTTCGCGTCGTGC | 59.66 | 58.77 | 201 | 127 |
100.00%
|
50.00%
|
| Primer 7 | Thermotoga petrophila | AGACACGCACACGCATCT | CGACGGCAACCACCTTTTC | 59.66 | 59.72 | 263 | 127 |
100.00%
|
50.00%
|
| Primer 8 | Thermotoga petrophila | AGCGCAACAGGTCACAGT | ATGGCACCTTCCATCGCA | 59.49 | 59.32 | 239 | 127 |
100.00%
|
50.00%
|
| Primer 9 | Thermotoga petrophila | ACAACGTGTCCGCACTGT | AGCCGTTCTGCTGCATCT | 59.81 | 59.33 | 151 | 127 |
100.00%
|
100.00%
|
| Primer 10 | Thermotoga petrophila | CTCGAGCGAGAACCCGATAG | AGGGGATTGGGGAGGAGAT | 59.76 | 58.95 | 260 | 127 |
100.00%
|
100.00%
|